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Karakterisering van eiwitten door grootte-uitsluiting chromatografie gekoppeld aan multi-angle lichtverstrooiing (SEC-MALS)
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
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Karakterisering van eiwitten door grootte-uitsluiting chromatografie gekoppeld aan multi-angle lichtverstrooiing (SEC-MALS)

50,654 Views

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10:00 min

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June 20, 2019

DOI:

10.3791/59615-v

DOI:
10.3791/59615-v

•

10:00 min
June 20, 2019

•

92 Views
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1Wyatt Technology Corporation, 2Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science,The Hebrew University of Jerusalem, 3Danyel Biotech Ltd.

Summary

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Dit protocol beschrijft de combinatie van grootte uitsluiting chromatografie met multi-angle lichtverstrooiing (SEC-MALS) voor absolute karakterisering van eiwitten en complexen in oplossing. SEC-MALS bepaalt het molecuulgewicht en de grootte van zuivere eiwitten, native oligomeren, heterocomplexes en gemodificeerde eiwitten zoals glycoproteïnen.

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Cite this Article

Some, D., Amartely, H., Tsadok, A., Lebendiker, M. Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS). J. Vis. Exp. (148), e59615, doi:10.3791/59615 (2019).

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