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从骨骼肌活检程序分离、培养、表征和分化人类肌肉祖细胞
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发育生物学
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JoVE 杂志 发育生物学
Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure
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从骨骼肌活检程序分离、培养、表征和分化人类肌肉祖细胞

8,080 Views

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00:08 min

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August 23, 2019

DOI:

10.3791/59580-v

DOI:
10.3791/59580-v

•

00:08 min
August 23, 2019

•

8 Views
, , , , ,
1Division of Nutritional Sciences,Cornell University, 2Meinig School of Biomedical Engineering,Cornell University

Summary

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我们介绍从骨骼肌活检组织获得的分离、培养、特征化和区分人类原发性肌肉祖细胞（hMPCs）的技术。通过这些方法获得和特征化的hMC可用于随后解决与人类肌发生和骨骼肌再生相关的研究问题。

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Cite this Article

Gheller, B. J., Blum, J., Soueid-Baumgarten, S., Bender, E., Cosgrove, B. D., Thalacker-Mercer, A. Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure. J. Vis. Exp. (150), e59580, doi:10.3791/59580 (2019).

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