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Detectando e caracterizando a auto-montagem da proteína in vivo por citometria de fluxo
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Detectando e caracterizando a auto-montagem da proteína in vivo por citometria de fluxo

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05:58 min

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July 17, 2019

DOI:

10.3791/59577-v

DOI:
10.3791/59577-v

•

05:58 min
July 17, 2019

•

7 Views
, , , ,
1Stowers Institute for Medical Research, 2Department of Molecular and Integrative Physiology,The University of Kansas School of Medicine

Summary

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Este artigo descreve um protocolo fret-based do citometria do fluxo para quantificar o Self-assembly da proteína em células de S. cerevisiae e de HEK293T.

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Cite this Article

Venkatesan, S., Kandola, T. S., Rodríguez-Gama, A., Box, A., Halfmann, R. Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry. J. Vis. Exp. (149), e59577, doi:10.3791/59577 (2019).

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