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मानव एंटीजन-विशिष्ट CD4+ टी कोशिकाओं का परिमाणीकरण Carboxyfluorescein Succinimidyl एस्टर का उपयोग कर
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Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester
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मानव एंटीजन-विशिष्ट CD4+ टी कोशिकाओं का परिमाणीकरण Carboxyfluorescein Succinimidyl एस्टर का उपयोग कर

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07:00 min

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June 04, 2019

DOI:

10.3791/59545-v

DOI:
10.3791/59545-v

•

07:00 min
June 04, 2019

•

8 Views
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1Immunology and Diabetes Unit,St. Vincent’s Institute of Medical Research, 2Department of Medicine,University of Melbourne, St. Vincent’s Hospital

Tags

CD4+ T CellsProliferationCFSEAntigen-specificQuantificationAutoimmune ConditionsType 1 DiabetesPBMCDensity Gradient CentrifugationCell CultureFlow Cytometry

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Di Carluccio, A. R., Tresoldi, E., So, M., Mannering, S. I. Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester. J. Vis. Exp. (148), e59545, doi:10.3791/59545 (2019).

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