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从细菌人工染色体cDNA克隆中拯救重组寨卡病毒
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
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从细菌人工染色体cDNA克隆中拯救重组寨卡病毒

26,251 Views

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08:10 min

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June 24, 2019

DOI:

10.3791/59537-v

DOI:
10.3791/59537-v

•

08:10 min
June 24, 2019

•

5 Views
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1Department of Microbiology and Immunology,University of Rochester Medical Center, 2Department of Molecular and Cell Biology,Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

Summary

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寨卡病毒最近的流行突出表明,必须建立反向遗传方法,以开发疫苗和/或治疗战略。在这里,我们描述了从全长cDNA克隆中拯救传染性重组寨卡病毒的协议,该克隆在人类细胞巨细胞病毒立即-早期启动子的控制下组装在细菌人工染色体中。

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Zika VirusReverse GeneticsBacterial Artificial ChromosomeCDNA CloneInfectious CloneRNA VirusFlavivirusViral BiologyViral PathogenesisVaccine DevelopmentTransfectionVero Cells

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Cite this Article

Ávila-Pérez, G., Park, J., Nogales, A., Almazán, F., Martínez-Sobrido, L. Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone. J. Vis. Exp. (148), e59537, doi:10.3791/59537 (2019).

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