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An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause
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生物化学
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JoVE 杂志 生物化学
An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause

An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause

DOI:
10.3791/59536-v

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06:18 min

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August 13, 2019

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, ,
1Institute of Medicinal Plant Development (IMPLAD),Chinese Academy of Medical Sciences & Peking Union Medical College, 2Shandong University of Traditional Chinese Medicine

Chapters

  • 00:05Title
  • 00:52Bilateral Ovariectomy
  • 02:22Body Weight, Total Cholesterol (TC), and Triglyceride (TG) Measurement
  • 03:25Aorta Harvest
  • 04:29Results: Representative Effects of Exogenous Estrogens on OVX Mouse Phenotype and Function
  • 05:46Conclusion

Summary

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自动翻译

Clinically, estrogen deficiency in menopausal women may aggravate the incidence of lipid disruption and atherosclerosis. We established an in vivo estrogen deficiency model by bilateral ovariectomy via a double dorsal-lateral incision in apoE-/- mice. The mouse model is applicable for screening exogenous estrogen treatments of cardiovascular dysfunction after menopause.

Tags

Keywords: OvariectomyEstrogen DeficiencyAPOE Knockout MiceCardiovascular DysfunctionMenopauseArteriosclerosisExogenous Estrogen TreatmentsPlasma CholesterolTriglycerides

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