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Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy
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生物化学
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JoVE 杂志 生物化学
Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy

DOI:
10.3791/59380-v

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09:43 min

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August 13, 2019

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, ,
1State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering,Southeast University

Chapters

  • 00:04Title
  • 01:07Preparation and Evaluation of Poly(2-Hydroxyethyl Methacrylate) (PHEMA) Hydrogels
  • 03:16Preparation of Silk Fibroin Solution and Evaluation of Secondary Structure Formation
  • 04:54Preparation of Short-Chain Oligonucleotide Duplex and Evaluation of Surrounding Water Molecules
  • 07:00Results: Selective Interface Evaluation with Sum Frequency Generation (SFG) Vibrational Spectroscopy
  • 08:31Conclusion

Summary

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Being comprehensively utilized, sum frequency generation (SFG) vibrational spectroscopy can help to reveal chain conformational order and secondary structural change happening at polymer and biomacromolecule interfaces.

Tags

Keywords: Sum Frequency GenerationVibrational SpectroscopyInterfacial StructuresPolymersBiomacromoleculesFresnel CoefficientSolid-liquid InterfaceFilm PreparationPHEMA

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