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Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants
JoVE 杂志
环境科学
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JoVE 杂志 环境科学
Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants

Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants

DOI:

08:58 min

July 29, 2019

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Chapters

  • 00:04Title
  • 01:16Culture E. coli OP50 and C. elegans
  • 02:00Prepare Synchronized Eggs and L3 Larvae of C. elegans
  • 04:12Use C. elegans for Trans-generational Effect Study
  • 05:58Use C. elegans for Multi-generational Exposure (MGE) Effect Study
  • 07:04Results: Trans-generational (TG), Multi-generational Exposure (MGE) and Multi-generational Residual (MGR) Effect Studies
  • 08:08Conclusion

Summary

自动翻译

Trans- and multi-generational effects of persistent chemicals are essential in judging their long-term consequences in the environment and on the human health. We provide novel detailed methods for studying trans- and multi-generational effects using free-living nematode Caenorhabditis elegans.

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