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Daily Phototherapy with Red Light to Regulate Candida albicans Biofilm Growth
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Daily Phototherapy with Red Light to Regulate Candida albicans Biofilm Growth

Daily Phototherapy with Red Light to Regulate Candida albicans Biofilm Growth

DOI:
10.3791/59326-v

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11:06 min

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April 23, 2019

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, ,
1Department of Cariology, Operative Dentistry and Dental Public Health,Indiana University School of Dentistry, 2Department of Restorative Dentistry,Ceará Federal University, School of Dentistry

Chapters

  • 00:04Title
  • 01:45Preparation of Culture Media
  • 03:22Pre-inoculum and Inoculum
  • 04:59Biofilm Formation and Phototherapy
  • 06:46Processing
  • 08:29Results: Analysis of CFU, Dry Weight, and EPS-soluble/insoluble Content of C. albicans Biofilm
  • 09:35Conclusion

Summary

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Here, we present a protocol to assess the outcome of red light application on the growth of Candida albicans biofilm. A non-coherent red light device with the wavelength of 635 nm and energy density of 87.6 J·cm-2 was applied throughout the growth of Candida albicans biofilms for 48 h.

Tags

Candida AlbicansBiofilmPhototherapyRed LightDisseminated Fungal InfectionExtracellular MatrixSpectrophotometerSDA MediumYNB MediumRPMI Medium

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