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Purification of the Dendritic Filopodia-rich Fraction
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神经科学
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JoVE 杂志 神经科学
Purification of the Dendritic Filopodia-rich Fraction

Purification of the Dendritic Filopodia-rich Fraction

DOI:
10.3791/59292-v

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11:51 min

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May 02, 2019

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,
1Laboratory for Neurobiology of Synapse,RIKEN Brain Science Institute, 2Liver Cancer Prevention Research Unit,RIKEN Center for Integrative Medical Sciences, 3Laboratory for Systems Molecular Ethology,RIKEN Center for Brain Science

Chapters

  • 00:04Title
  • 00:35Preparation of Culture Medium and Poly-L-Lysine-coated Dishes
  • 04:58Preparation of Hippocampal Neurons
  • 07:04Purification of Dendritic Filopodia-rich Fraction, Silver Staining and Western Blot Analysis
  • 09:33Results: Purification Method for the Dendritic Filopodia-rich Fraction
  • 11:12Conclusion

Summary

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In this protocol, we introduce a method for purifying the dendritic filopodia-rich fraction from the phagocytic cup-like protrusion structure on cultured hippocampal neurons by taking advantage of the specific and strong affinity between a dendritic filopodial adhesion molecule, TLCN, and an extracellular matrix molecule, vitronectin.

Tags

Keywords: Dendritic FilopodiaSynaptic ProteinImmature SynapsePhagocytic Cap FormationArtificial GenesisVitamin MixRiboflavin SolutionCalcium DichlorideMinimum Essential Medium (MEM)DNase-1Ara-CPlating Medium

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