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分離、伝達、およびプリオン蛋白質発現ひと歯髄幹細胞の神経分化
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
DOI:

11:50 min

March 18, 2019

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Chapters

  • 00:04Title
  • 00:47Tooth and Dental Pulp Extraction
  • 01:29Processing of the Dental Pulp and Stem Cell Release
  • 02:29Stem Cell Culture and Propagation
  • 03:44Transient PrPC Silencing by siRNA
  • 05:04Neuronal Induction Process of hDPSCs
  • 05:50Characterization of hDPSCs by Flow Cytometry
  • 08:03Evaluation of PrPC Expression in hDPSCs by Flow Cytometry Analysis
  • 09:14Results: hDPSCs Isolation and Propagation and the Role of PrP<supC During Neuronal Differentiation
  • 10:54Conclusion

Summary

自动翻译

ここでプリオン蛋白発現神経細胞の分化過程を評価するために人間の歯髄幹細胞の分離と伝搬するためのプロトコルを提案する.

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