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人牙髓干细胞神经分化过程中的分离、繁殖及普利翁蛋白表达
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
DOI:

11:50 min

March 18, 2019

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Chapters

  • 00:04Title
  • 00:47Tooth and Dental Pulp Extraction
  • 01:29Processing of the Dental Pulp and Stem Cell Release
  • 02:29Stem Cell Culture and Propagation
  • 03:44Transient PrPC Silencing by siRNA
  • 05:04Neuronal Induction Process of hDPSCs
  • 05:50Characterization of hDPSCs by Flow Cytometry
  • 08:03Evaluation of PrPC Expression in hDPSCs by Flow Cytometry Analysis
  • 09:14Results: hDPSCs Isolation and Propagation and the Role of PrP<supC During Neuronal Differentiation
  • 10:54Conclusion

Summary

自动翻译

本文提出了一种人类牙髓干细胞分离和繁殖的方案, 以评估神经元分化过程中的 prion 蛋白表达。

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