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Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique
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JoVE 杂志 遗传学
Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

5,253 Views

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05:24 min

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April 01, 2019

DOI:

10.3791/59213-v

DOI:
10.3791/59213-v

•

05:24 min
April 01, 2019

•

2 Views
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1Graduate Interdisciplinary Program in Entomology and Insect Science,University of Arizona, 2Department of Entomology,University of Arizona

Summary

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We present a simple cytogenetic technique using 4′,6-diamidino-2-phenylindole (DAPI) to determine the fertilization rate and primary sex ratio of the haplodiploid invasive pest Bemisia tabaci.

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Bemisia TabaciWhiteflyCytogenetic TechniqueEgg Fertilization RateHaplodiploidSex RatioOvipositionDechorionationDAPI StainingMicroscopy

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Cite this Article

Bondy, E. C., Hunter, M. S. Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique. J. Vis. Exp. (146), e59213, doi:10.3791/59213 (2019).

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