需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
10,239 Views
•
09:21 min
•
February 15, 2019
DOI:
Summary
Automatically generated
Here we present a protocol to analyze Aspergillus flavus growth and aflatoxin production in maize kernels expressing an antifungal protein. Using a GFP-expressing A. flavus strain we monitored the infection and spread of the fungus in mature kernels in real time. The assay is rapid, reliable, and reproducible.
Read Article
Cite this Article
Rajasekaran, K., Sayler, R. J., Majumdar, R., Sickler, C. M., Cary, J. W. Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.. J. Vis. Exp. (144), e59169, doi:10.3791/59169 (2019).
Copy