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1Department of Plant Ecology,Forestry and Forest Products Research Institute (FFPRI), 2Kansai Research Center,Forestry and Forest Products Research Institute (FFPRI), 3Department of Wood Properties and Processing,Forestry and Forest Products Research Institute (FFPRI), 4Faculty of Agriculture,Kyushu University, 5Department of Bacteriology,University of Wisconsin, 6Research Faculty of Agriculture,Hokkaido University
Chapters
- 00:04Title
- 01:14Sampling: Tension Relaxation within Water Column of Xylem Conduits
- 03:00Freeze Fixation with Liquid Nitrogen
- 04:04Specimen Preparation
- 05:49Transfer to the Cryo-SEM Specimen Chamber
- 06:30Freeze-etching
- 07:00Results: Xylem Water Distribution in Woody Plants
- 09:04Conclusion
Het observeren van de waterdistributie binnen de Xylem geeft belangrijke informatie over de Water stromings dynamiek in bosrijke planten. In deze studie demonstreren we de praktische aanpak om Xylem waterdistributie in situ te observeren met behulp van een cryostat en Cryo-SEM, die artefactuele veranderingen in de water status elimineert tijdens de monstervoorbereiding.
Tags
XylemWater DistributionCryo-scanning Electron MicroscopeFreeze-fixationXylem TensionWater PotentialCavitationXylem CellsAerotomeWoody PlantsWater RegimeSeasonal VariationBiotic StressFreeze/thaw CycleWet WoodSampling ProcedurePressure ChamberRelaxation TreatmentWater SurfaceTranspirationVessel Length
