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ミリ秒時間スケールでのタンパク質複合体のダイナミクスを調べるため体外蛍光共鳴エネルギー移動の使用
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生物化学
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JoVE 杂志 生物化学
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
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ミリ秒時間スケールでのタンパク質複合体のダイナミクスを調べるため体外蛍光共鳴エネルギー移動の使用

7,870 Views

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10:50 min

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March 14, 2019

DOI:

10.3791/59038-v

DOI:
10.3791/59038-v

•

10:50 min
March 14, 2019

•

2 Views
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1Department of Biochemistry,Purdue University, 2Department of Botany and Plant Pathology,Purdue University, 3Center for Plant Biology,Purdue University

Tags

FRETProtein ComplexKineticsAssociationDissociationCOL1CAND17-amino-4-methylcoumarinFlASHCell LysisSonicationCentrifugationGlutathione BeadsElutionThrombinBuffer A

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Garsamo, M., Zhou, Y., Liu, X. Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale. J. Vis. Exp. (145), e59038, doi:10.3791/59038 (2019).

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