Journal
/
环境科学
/
Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
JoVE 杂志
环境科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 环境科学
Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

8,439 Views

•

09:38 min

•

January 07, 2019

DOI:

10.3791/59035-v

DOI:
10.3791/59035-v

•

09:38 min
January 07, 2019

•

1 Views
, , , , , , ,
1Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory, 2Department of Soil, Water and Environmental Science,University of Arizona, 3Department of Earth Ocean and Atmospheric Sciences,Florida State University, 4Bruker Daltonics Inc., 5Biological Sciences Division,Pacific Northwest National Laboratory

Summary

Automatically generated

This protocol describes a single throughput for complementary analytical and omics techniques culminating in a fully-paired characterization of natural organic matter and microbial proteomics in different ecosystems. This approach permits robust comparisons for identifying metabolic pathways and transformations important for describing greenhouse gas production and predicting responses to environmental change.

Explore More Videos

Natural Organic MatterMulti-omicsMass SpectrometryNMR SpectroscopyMetabolomicsProteomicsLipidomicsSequential ExtractionMPLEx ExtractionBioavailable CompoundsPolar CompoundsNon-polar CompoundsSample Preparation

Read Article

Cite this Article

Tfaily, M. M., Wilson, R. M., Brewer, H. M., Chu, R. K., Heyman, H. M., Hoyt, D. W., Kyle, J. E., Purvine, S. O. Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures. J. Vis. Exp. (143), e59035, doi:10.3791/59035 (2019).

Download .ris file

Copy

Share Video

.

Copy