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利用自动高通量微流控系统对铜绿假单胞菌剪切应力的活细胞分析
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January 16, 2019
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在这里, 我们描述了使用高通量微流体生物反应器结合荧光显微镜来分析剪切应力对表达绿色荧光蛋白的铜绿假单胞菌生物膜的剪切应力影响, 包括仪器建立了生物膜覆盖、生长速率和形态特性的测定。
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Sutlief, A. L., Valquier-Flynn, H., Wilson, C., Perez, M., Kleinschmidt, H., Schofield, B. J., Delmain, E., Holmes, A. E., Wentworth, C. D. Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System. J. Vis. Exp. (143), e58926, doi:10.3791/58926 (2019).
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