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Novel Sequence Discovery by Subtractive Genomics
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Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

DOI:
10.3791/58877-v

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09:40 min

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January 25, 2019

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, ,
1Biology Department,American University

Chapters

  • 00:04Title
  • 01:08De novo Assemble Starting Sequence
  • 02:03BLAST the Assembly Against the Reference Sequence
  • 04:44Design Primers for the Sequence that Remains
  • 06:25qPCR Validation of the Remaining Sequence
  • 07:51Results: Example Data of qPCR Results Including Negative and Positive Outcomes
  • 08:53Conclusion

Summary

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The purpose of this protocol is to use a combination of computational and bench research to find novel sequences that cannot be easily separated from a co-purifying sequence, which may be only partially known.

Tags

Novel Sequence DiscoverySubtractive GenomicsGenomic SequencesInexpensiveFlexibleBacterial Vaccine TargetsVirus IdentificationUnknown SeparationReference SequenceTrimmomaticPearReptileTrinityBlastTransdeckorTblastn

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