需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
Chapters
- 00:04Title
- 00:29Circular DNA Extraction
- 02:17Assembly Solution Annealing
- 04:53Finite Lattice Purification
- 06:17Atomic Force Microscopy (AFM) Imaging
- 07:58Results: Representative 1D and 2D DNA Lattice Analyses
- 08:55Conclusion
This article presents a detailed protocol for T4 ligation and denaturing PAGE purification of small circular DNA molecules, annealing and native PAGE analysis of circular tiles, assembling and AFM imaging of 1D and 2D DNA nanostructures, as well as agarose gel electrophoresis and centrifugation purification of finite DNA nanostructures.
