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Bepalende 3'-Termini en sequenties van ontluikende Single-Stranded virale DNA moleculen tijdens HIV-1 Reverse transcriptie in geïnfecteerde cellen
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Determining 3′-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
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Bepalende 3'-Termini en sequenties van ontluikende Single-Stranded virale DNA moleculen tijdens HIV-1 Reverse transcriptie in geïnfecteerde cellen

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13:07 min

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January 30, 2019

DOI:

10.3791/58715-v

DOI:
10.3791/58715-v

•

13:07 min
January 30, 2019

•

2 Views
, ,
1Department of Infectious Diseases, School of Immunology & Microbial Sciences,King’s College London

Summary

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Hier presenteren we een diepe rangschikken benadering waarmee een onbevooroordeelde bepaling van ontluikende 3'-termini en vogelgriepvirus profielen van single-stranded DNA moleculen. De belangrijkste toepassing is de karakterisatie van ontluikende retrovirale complementaire Ani (cDNAs), de tussenproducten die tijdens het proces van retrovirale omgekeerde transcriptie gegenereerd.

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Pollpeter, D., Sobala, A., Malim, M. H. Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells. J. Vis. Exp. (143), e58715, doi:10.3791/58715 (2019).

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