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用不连续密度梯度用胶囊分离细菌

JoVE 杂志
遗传学

This content is Free Access.

JoVE 杂志遗传学

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

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用不连续密度梯度用胶囊分离细菌

13,273 Views

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05:52 min

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January 07, 2019

DOI:

10.3791/58679-v

DOI:

10.3791/58679-v

•

05:52 min

January 07, 2019

•

11 Views

Theresa Feltwell¹, Matthew J. Dorman¹, David A. Goulding¹, Julian Parkhill¹, Francesca L. Short^1,2

¹Wellcome Sanger Institute, Wellcome Genome Campus, ²Department of Medicine,University of Cambridge

Summary

我们展示了使用不连续密度梯度来根据胶囊生产分离细菌种群。该方法用于比较培养物间的胶囊数量, 分离具有特定胶囊表型的突变体, 或鉴定胶囊调节剂。这里描述的是该分析的优化和运行。

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