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Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
Chapters
- 00:04Title
- 00:33Cell Culture, and the Collection of EVs and RNA-associated Biomolecules
- 02:54RNA Extraction and Quality Control
- 04:20Library Construction and Quality Control
- 09:04Results: Collecting exRNA from hMSC for Next Generation Sequencing
- 10:31Conclusion
This protocol demonstrates how to purify extracellular microRNAs from cell culture media for small RNA library construction and next generation sequencing. Various quality control checkpoints are described to allow readers to understand what to expect when working with low input samples like exRNAs.
