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Journal / Biochemistry / Isolation of Tissue Extracellular Vesicles from…
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Biochemistry

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JoVE Journal Biochemistry
Isolation of Tissue Extracellular Vesicles from the Liver
 

Isolation of Tissue Extracellular Vesicles from the Liver

Article DOI: 10.3791/58649-v • 05:39 min • August 21st, 2019
August 21st, 2019 •
1, 1, 1
1Department of Transplantation, Mayo Clinic

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总结概括
Automatic Translation

This is a protocol to isolate tissue extracellular vesicles (EVs) from the liver. The protocol describes a two-step process involving collagenase perfusion followed by differential ultracentrifugation to isolate liver tissue EVs.

Tags

Keywords: Extracellular Vesicles Liver Tissue Microenvironment Portal Vein Cannulation HBSS Perfusion Collagenase Digestion Tissue Isolation
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