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Journal / Biology / 基于细胞健康监测的实时细胞成像分析对低密度脂蛋白胆固醇的吸收检测…
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基于细胞健康监测的实时细胞成像分析对低密度脂蛋白胆固醇的吸收检测
 
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基于细胞健康监测的实时细胞成像分析对低密度脂蛋白胆固醇的吸收检测

Article DOI: 10.3791/58564-v • 08:45 min • November 17th, 2018
November 17th, 2018 •
*1,2, *1,3, 4, 4,5, 6, 1,2,7,8
1Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 2Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 3Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 4Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Leonard M. Miller School of Medicine, 5John P. Hussman Institute for Human Genomics, University of Miami Leonard M. Miller School of Medicine, 6Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 7Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 8Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine
* These authors contributed equally

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该协议提供了一种有效的方法来测量低密度脂蛋白胆固醇的吸收与实时流入率使用活细胞成像系统在不同的细胞类型。该技术提供了一个平台, 以筛选影响低密度脂蛋白流入的化合物的药理活性, 同时监测细胞形态, 从而潜在的细胞毒性。

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生物学 第141期 低密度脂蛋白 ldlr 低密度脂蛋白流入 低密度脂蛋白吸收 dynasore 辛伐他汀 重组 pcsk9 动力 脂质 胆固醇 动脉粥样硬化。
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