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Meten van interacties van bolvormige en filamenteuze eiwitten door nucleaire magnetische resonantie spectroscopie (NMR) en Microscale Thermophoresis (MST)
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
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Meten van interacties van bolvormige en filamenteuze eiwitten door nucleaire magnetische resonantie spectroscopie (NMR) en Microscale Thermophoresis (MST)

11,734 Views

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10:28 min

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November 02, 2018

DOI:

10.3791/58537-v

DOI:
10.3791/58537-v

•

10:28 min
November 02, 2018

•

11 Views
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1Department of Biochemistry,University of Alberta

Summary

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Hier presenteren we een protocol voor de productie en zuivering van eiwitten die zijn gemarkeerd met stabiele isotopen, en verdere karakterisering van eiwit-eiwitinteractie met behulp nucleaire magnetische resonantie (NMR) spectroscopie en MicroScale Thermophoresis (MST) experimenten.

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Winkelaar, G., Trieber, C., Kumar, J., Overduin, M. Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST). J. Vis. Exp. (141), e58537, doi:10.3791/58537 (2018).

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