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Nükleer manyetik rezonans spektroskopisi (NMR) ve Microscale Thermophoresis (MST) tarafından küresel ve ipliksi protein etkileşimleri ölçme
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
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Nükleer manyetik rezonans spektroskopisi (NMR) ve Microscale Thermophoresis (MST) tarafından küresel ve ipliksi protein etkileşimleri ölçme

11,739 Views

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10:28 min

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November 02, 2018

DOI:

10.3791/58537-v

DOI:
10.3791/58537-v

•

10:28 min
November 02, 2018

•

11 Views
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1Department of Biochemistry,University of Alberta

Summary

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Burada, üretim ve arıtma etiketlenir proteinlerin için bir protokol kararlı izotop ve protein-protein etkileşimleri nükleer manyetik rezonans (NMR) spektroskopisi ve MicroScale kullanarak sonraki karakterizasyonu ile mevcut Thermophoresis (MST) deneyler.

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Cite this Article

Winkelaar, G., Trieber, C., Kumar, J., Overduin, M. Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST). J. Vis. Exp. (141), e58537, doi:10.3791/58537 (2018).

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