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Isolation, Fixation, and Immunofluorescence Imaging of Mouse Adrenal Glands
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JoVE 杂志 免疫与感染
Isolation, Fixation, and Immunofluorescence Imaging of Mouse Adrenal Glands

Isolation, Fixation, and Immunofluorescence Imaging of Mouse Adrenal Glands

DOI:
10.3791/58530-v

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08:37 min

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October 02, 2018

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,
1Department of Internal Medicine (Division of Metabolism, Endocrinology, and Diabetes),University of Michigan Health System, 2Department of Cell and Developmental Biology,University of Michigan Health System, 3Department of Molecular and Integrative Physiology,University of Michigan Health System, 4Endocrine Oncology Program,University of Michigan Health System, 5Comprehensive Cancer Center,University of Michigan Health System

Chapters

  • 00:04Title
  • 00:31Adrenal Dissection, Processing, and Embedding
  • 02:36Rotary Microtome Sectioning
  • 04:06Immunofluorescence
  • 06:57Results: Representative Adrenal Gland Imaging and Analysis
  • 07:46Conclusion

Summary

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Here we present a method to isolate adrenal glands from mice, fix the tissues, section them, and perform immunofluorescence staining.

Tags

ImmunofluorescenceAdrenal GlandMouseIsolationFixationParaffin EmbeddingMicrotome Sectioning

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