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Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines

Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines

11,531 Views

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05:39 min

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October 30, 2018

DOI:

10.3791/58453-v

DOI:
10.3791/58453-v

•

05:39 min
October 30, 2018

•

6 Views
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1BioMediTech Institute, Faculty of Medicine and Life Sciences,University of Tampere, 2MedEngine Oy, 3Department of Pediatrics,Tampere University Hospital, 4Department of Children and Adolescents,Oulu University Hospital, 5PEDEGO Research Unit, Medical Research Center,University of Oulu

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Here we describe a protocol for the immunization of the adult zebrafish (Danio rerio) with a DNA-based vaccine and demonstrate the validation of a successful vaccination event. This method is suitable for the preclinical screening of vaccine candidates in various infection models.

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Myllymäki, H., Niskanen, M., Oksanen, K., Rämet, M. Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines. J. Vis. Exp. (140), e58453, doi:10.3791/58453 (2018).

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