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Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
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生物学
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JoVE 杂志 生物学
Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

DOI:
10.3791/58431-v

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07:39 min

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January 07, 2019

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, , , ,
1Leicester Cancer Research Centre and Ernest and Helen Scott Haematology Research Institute,University of Leicester, 2MRC Toxicology Unit,University of Leicester

Chapters

  • 00:04Title
  • 00:43CD4+ T Cell Peripheral Whole Blood Isolation and Flow Cytometric Analysis
  • 04:45Data Analysis
  • 06:07Results: Representative Circulating T-follicular Helper (cTfh) Cell Subset Analyses
  • 06:53Conclusion

Summary

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Here, a protocol for the isolation and characterization of CD4+ T-cell subsets from human peripheral blood is described. Purified CD4+ T-cells are analyzed by flow cytometry to determine proportions of T-follicular helper cell subsets.

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Keywords: CD4+ T-cellsT-follicular Helper (Tfh) CellsCirculating T-follicular Helper (cTfh) CellsFlow CytometryBiomarkerAutoimmune DiseaseB-cell Non-Hodgkin’s LymphomaPeripheral BloodCell IsolationCell Subsets

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