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In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice

In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice

11,739 Views

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06:24 min

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October 18, 2018

DOI:

10.3791/58340-v

DOI:
10.3791/58340-v

•

06:24 min
October 18, 2018

•

14 Views
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1International Research Center for Medical Sciences (IRCMS),Kumamoto University, 2Division of Neurogenetics,National Institute of Genetics, 3Department of Genetics,SOKENDAI (The Graduate University for Advanced Studies)

Summary

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We present an in vivo two-photon imaging protocol for imaging the cerebral cortex of neonatal mice. This method is suitable for analyzing the developmental dynamics of cortical neurons, the molecular mechanisms that control the neuronal dynamics, and the changes in neuronal dynamics in disease models.

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Mizuno, H., Nakazawa, S., Iwasato, T. In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice. J. Vis. Exp. (140), e58340, doi:10.3791/58340 (2018).

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