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Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues
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环境科学
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JoVE 杂志 环境科学
Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues

Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues

DOI:
10.3791/58232-v

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07:05 min

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October 04, 2018

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, , , , , , ,
1Graduate Institute of Molecular and Comparative Pathobiology,National Taiwan University, 2School of Veterinary Medicine,National Taiwan University, 3Department of Oceanography and Asia-Pacific Ocean Research Center,National Sun Yat-sen University, 4Graduate Institute of Veterinary Pathobiology,National Chung Hsing University

Chapters

  • 00:04Title
  • 00:57Autometallography Analysis Tissue Sample Preparation
  • 03:48Semi-Quantitative AMG Analysis and Cetacean Histological Ag Assay (CHAA) by Regression
  • 05:54Results: Representative Histological Images of AMG Positive Signals in the Liver and Kidney Tissues of Stranded Cetaceans
  • 06:35Conclusion

Summary

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A protocol is presented to localize Ag in cetacean liver and kidney tissues by autometallography. Furthermore, a new assay, named the cetacean histological Ag assay (CHAA) is developed to estimate the Ag concentrations in those tissues.

Tags

AutometallographySilverEnvironmental ToxicologyHeavy MetalsLiverKidneyFormalin FixationParaffin EmbeddingMicrotomeDe-paraffinationHydrationPBSTriton X-100

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