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1School of Laboratory Medicine and Life Sciences,Wenzhou Medical University, 2NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, 3State Key Laboratory of Agrobiotechnology, College of Biological Sciences,China Agricultural University, 4Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science,Chinese Academy of Sciences, 5Laboratory Animal Center,Chinese Center for Disease Control and Prevention, 6CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology,Chinese Academy of Sciences
Chapters
- 00:04Title
- 01:15Zika Virus (ZIKV) Infection, Immunization, and Splenic Monocyte Isolation
- 03:19Brain and Testicular Monocyte Isolation
- 06:10Flow Cytometry
- 07:26Results: ZIKV Infection Characterization in Ifnar1-/- Mice
- 09:10Conclusion
Se describe un protocolo para evaluar las respuestas de células T antígeno específicas en los órganos de immunoprivileged del Ifnar1- / - modelo murino para la infección del virus (ZIKV) Zika. Este método es fundamental para la investigación de los mecanismos celulares de la protección y la inmunopatogénesis de las vacunas ZIKV y también es valiosa para la evaluación de su eficacia.
Tags
Zika VirusT-cell ResponsesImmunoprivileged OrgansIfnar1-/- MiceTetramer StainingImmunopathogenesisGuillain-Barre SyndromeTestes InjuryInterferon Alpha/beta Receptor KnockoutBrainTestesSpleenFocus-forming UnitsRetro-orbital InoculationAbdominal SurgerySingle-cell SuspensionRed Blood Cell LysisFlow Cytometry
