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用快速 Bioorthogonal 反应生成抗体-药物共轭物的非规范氨基酸的遗传编码
JoVE 杂志
化学
This content is Free Access.
JoVE 杂志 化学
Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction
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用快速 Bioorthogonal 反应生成抗体-药物共轭物的非规范氨基酸的遗传编码

7,481 Views

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11:02 min

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September 14, 2018

DOI:

10.3791/58066-v

DOI:
10.3791/58066-v

•

11:02 min
September 14, 2018

•

10 Views
1Medical Research Council Laboratory of Molecular Biology

Summary

Automatically generated

将赖氨酸的 cyclopropene 衍生物加入抗体, 就能使四嗪类分子的特异性、快速和有效的联系产生抗体-药物共轭。

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Cite this Article

Oller-Salvia, B. Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction. J. Vis. Exp. (139), e58066, doi:10.3791/58066 (2018).

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