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Caracterização de subconjuntos de monócitos humanos por sangue total Flow Cytometry Analysis
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Caracterização de subconjuntos de monócitos humanos por sangue total Flow Cytometry Analysis

56,510 Views

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09:12 min

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October 17, 2018

DOI:

10.3791/57941-v

DOI:
10.3791/57941-v

•

09:12 min
October 17, 2018

•

37 Views
, , , , ,
1Department of Surgery, Vascular Biology Research Centre,Westmead Hospital, 2Westmead Clinical School, Department of Surgery,The University of Sydney, 3Westmead Research Hub,Westmead Institute for Medical Research, 4Institute for Clinical Pathology and Medical Research,Westmead Hospital

Summary

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Aqui nós apresentamos um protocolo para caracterizar subconjuntos de monócitos por citometria de fluxo de sangue total. Isso inclui como portão os subconjuntos e avaliar sua expressão de marcadores de superfície de estrutura de tópicos e dando um exemplo da avaliação da expressão de marcadores M2 (anti-inflamatório) e M1 (inflamatória).

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Cite this Article

Marimuthu, R., Francis, H., Dervish, S., Li, S. C., Medbury, H., Williams, H. Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis. J. Vis. Exp. (140), e57941, doi:10.3791/57941 (2018).

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