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免疫与感染

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全血流式细胞术分析人单核细胞亚群的表征

JoVE 杂志
免疫与感染

This content is Free Access.

JoVE 杂志免疫与感染

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

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全血流式细胞术分析人单核细胞亚群的表征

56,510 Views

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09:12 min

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October 17, 2018

DOI:

10.3791/57941-v

DOI:

10.3791/57941-v

•

09:12 min

October 17, 2018

•

37 Views

Rekha Marimuthu^1,2, Habib Francis^1,2, Suat Dervish³, Stephen C.H. Li⁴, Heather Medbury*^1,2, Helen Williams*^1,2

¹Department of Surgery, Vascular Biology Research Centre,Westmead Hospital, ²Westmead Clinical School, Department of Surgery,The University of Sydney, ³Westmead Research Hub,Westmead Institute for Medical Research, ⁴Institute for Clinical Pathology and Medical Research,Westmead Hospital

Summary

在这里, 我们提出了一个通过全血流式细胞仪表征单核细胞子集的协议。这包括概述如何门的子集和评估他们的表面标记的表达, 并提供了一个例子, 评估 M1 (炎症) 和 M2 标记 (抗炎) 的表达。

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