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Environment

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JoVE Journal Environment
Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish
 

Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine

Article DOI: 10.3791/57938-v • 08:33 min • July 24th, 2018
July 24th, 2018 •
1,2, 1, 1,2
1Department of Environmental Science, Center for Reservoir and Aquatic Systems Research, Baylor University, 2Institute of Biomedical Studies, Baylor University

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Here, we present a protocol to examine larval zebrafish and fathead minnow locomotor activities and photomotor responses (PMR) using an automated tracking software. When incorporated in common toxicology bioassays, analyses of these behaviors provide a diagnostic tool to examine chemical bioactivity. This protocol is described using caffeine, a model neurostimulant.

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Keywords: Fish Models Behavioral Response Larval Fish Caffeine Neuro-stimulant Environmental Biomedical Sciences Locomotor Activity Photomotor Response Zebrafish Fathead Minnow Exposure Chambers Video Tracking
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