/

/

Correzione di bozze e saggio di riparazione del DNA usando l'analisi di spettrometria di massa di singolo Nucleotide estensione e MALDI-TOF

JoVE 杂志
生物化学

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志生物化学

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Please note that all translations are automatically generated. Click here for the English version.

Correzione di bozze e saggio di riparazione del DNA usando l'analisi di spettrometria di massa di singolo Nucleotide estensione e MALDI-TOF

9,402 Views

•

11:08 min

•

June 19, 2018

DOI:

10.3791/57862-v

DOI:

10.3791/57862-v

•

11:08 min

June 19, 2018

•

1 Views

Kang-Yi Su*^1,2, Steven D. Goodman*³, Hung-Ming Lai, Rong-Syuan Yen, Wei-Yao Hu, Wern-Cherng Cheng, Liang-In Lin², Ya-Chien Yang², Woei-Horng Fang²

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, ²Department of Laboratory Medicine,National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics,The Ohio State University

Tags

DNA Repair Proofreading MALDI-TOF Mass Spectrometry Single Nucleotide Extension DNA Fidelity DNA Polymerase Primer-template Preparation Mismatch Substrate Reaction Buffer DdNTPs DNA Polymerase Dilution Reaction Temperature Reaction Termination

Read Article