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Correction d’épreuves et de test de réparation d’ADN en utilisant l’analyse de spectrométrie de masse MALDI-TOF et de nucléotide simple Extension

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生物化学

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JoVE 杂志生物化学

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

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Correction d’épreuves et de test de réparation d’ADN en utilisant l’analyse de spectrométrie de masse MALDI-TOF et de nucléotide simple Extension

9,402 Views

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11:08 min

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June 19, 2018

DOI:

10.3791/57862-v

DOI:

10.3791/57862-v

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11:08 min

June 19, 2018

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1 Views

Kang-Yi Su*^1,2, Steven D. Goodman*³, Hung-Ming Lai, Rong-Syuan Yen, Wei-Yao Hu, Wern-Cherng Cheng, Liang-In Lin², Ya-Chien Yang², Woei-Horng Fang²

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, ²Department of Laboratory Medicine,National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics,The Ohio State University

Tags

DNA Repair Proofreading MALDI-TOF Mass Spectrometry Single Nucleotide Extension DNA Fidelity DNA Polymerase Primer-template Preparation Mismatch Substrate Reaction Buffer DdNTPs DNA Polymerase Dilution Reaction Temperature Reaction Termination

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