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单核苷酸延长和 MALDI 质谱分析校对及 DNA 修复方法

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生物化学

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JoVE 杂志生物化学

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

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单核苷酸延长和 MALDI 质谱分析校对及 DNA 修复方法

9,427 Views

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11:08 min

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June 19, 2018

DOI:

10.3791/57862-v

DOI:

10.3791/57862-v

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11:08 min

June 19, 2018

•

1 Views

Kang-Yi Su*^1,2, Steven D. Goodman*³, Hung-Ming Lai¹, Rong-Syuan Yen¹, Wei-Yao Hu¹, Wern-Cherng Cheng², Liang-In Lin^1,2, Ya-Chien Yang^1,2, Woei-Horng Fang^1,2

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, ²Department of Laboratory Medicine,National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics,The Ohio State University

Summary

采用高分辨率 MALDI 质谱和单核苷酸扩展策略, 建立了 dna 聚合酶校对和 dna 修复实验的非标记非射电同位素方法。试验证明是非常具体, 简单, 快速, 并易于执行校对和修复补丁短于 9-核苷酸。

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