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高频刺激激活小鼠齿状回的侵袭方法
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JoVE 杂志 神经科学
An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation
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高频刺激激活小鼠齿状回的侵袭方法

7,502 Views

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12:26 min

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June 02, 2018

DOI:

10.3791/57857-v

DOI:
10.3791/57857-v

•

12:26 min
June 02, 2018

•

6 Views
,
1Department of Neurobiology,Beijing Institute of Basic Medical Sciences, 2Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration,Nantong University

Summary

Automatically generated

该协议说明如何在小鼠中建立可靠的痉挛方法。整个海马齿状回的神经元由痉挛直接和间接的在体内电刺激。神经活动和分子信号由c-fos和 Notch1 免疫荧光染色分别检查;bromodeoxyuridine 标记法对神经发生进行量化。

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Cite this Article

Zhao, Z., Wu, H. An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation. J. Vis. Exp. (136), e57857, doi:10.3791/57857 (2018).

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