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高频刺激激活小鼠齿状回的侵袭方法
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation
DOI:

12:26 min

June 02, 2018

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Chapters

  • 00:04Title
  • 01:06Electrode Implantation
  • 05:24High-Frequency Stimulation Delivery
  • 06:56Immunofluorescence Staining and Bromodeoxyuridine Labeling
  • 10:05Results: Distinct activation of neurogenesis in the DG in response to HFS in DG and PP
  • 11:46Conclusion

Summary

自动翻译

该协议说明如何在小鼠中建立可靠的痉挛方法。整个海马齿状回的神经元由痉挛直接和间接的在体内电刺激。神经活动和分子信号由c-fos和 Notch1 免疫荧光染色分别检查;bromodeoxyuridine 标记法对神经发生进行量化。

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