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The Barnacle Balanus improvisus as a Marine Model – Culturing and Gene Expression
JoVE 杂志
环境科学
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JoVE 杂志 环境科学
The Barnacle Balanus improvisus as a Marine Model – Culturing and Gene Expression

The Barnacle Balanus improvisus as a Marine Model – Culturing and Gene Expression

14,188 Views

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07:47 min

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August 08, 2018

DOI:

10.3791/57825-v

DOI:
10.3791/57825-v

•

07:47 min
August 08, 2018

•

5 Views
, , , , ,
1Department of Marine Sciences,University of Gothenburg, 2Department of Chemistry and Molecular Biology,University of Gothenburg, 3IVL Swedish Environmental Research Institute

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The barnacle Balanus (Amphibalanus) improvisus is a model for studying osmoregulation and antifouling. However, natural seasonal spawning yields an unpredictable supply of cyprid larvae. Here, a protocol for the all-year-round culturing of B. improvisus is described, including the production of larvae. The use of cultured barnacles in gene expression studies is illustrated.

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Jonsson, P. R., Wrange, A., Lind, U., Abramova, A., Ogemark, M., Blomberg, A. The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression. J. Vis. Exp. (138), e57825, doi:10.3791/57825 (2018).

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