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生物工程
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人隐静脉的去细胞和 Recellularization 方法学研究
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Decellularization and Recellularization Methodology for Human Saphenous Veins
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人隐静脉的去细胞和 Recellularization 方法学研究

DOI:
10.3791/57803-v

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11:35 min

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July 27, 2018

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, ,
1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy,University of Gothenburg

Chapters

  • 00:04Title
  • 00:43Preparation of Decellularization Setups and Recellularization Bioreactor
  • 03:55Decellularization of Saphenous Vein
  • 06:14Recellularization
  • 09:22Results: Evaluation of Normal, Decellularized, and Recellularized Veins
  • 10:13Conclusion

Summary

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自动翻译

在这里, 我们描述了一个协议的隐静脉去细胞使用洗涤剂和 recellularization 的血液灌注的外周血和内皮介质。

Tags

Keywords: DecellularizationRecellularizationSaphenous VeinTriton-X 100PerfusionWashingBioreactorCell CultureBone MarrowTnBPDNAMedia InletMedia OutletVein InletVein OutletPeristaltic Pump

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