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A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions
JoVE 杂志
环境科学
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JoVE 杂志 环境科学
A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

DOI:
10.3791/57800-v

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11:27 min

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August 25, 2018

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, , , , , ,
1Brazilian Bioethanol Science and Technology Laboratory (CTBE),Brazilian Center for Research in energy and materials (CNPEM), 2University of Campinas (UNICAMP), 3University of Viçosa (UFV), 4CTBE,CNPEM, 5Brazilian Bioethanol Science and Technology Laboratory (CTBE/CNPEM),Max Planck Partner Group

Chapters

  • 00:00Title
  • 00:46Reagents
  • 01:02Equipments
  • 01:38Hydroponic system assembling
  • 01:50Material sterilization
  • 02:18Mini-tank assembling
  • 03:32Seed sterilization
  • 05:08Seed application
  • 05:57Validating the use of this hydroponic system to inhibit the Target Of Rapamycin kinase
  • 07:17Representative results and discussion
  • 10:46Conclusion

Summary

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A simple, versatile, and low-cost in vitro hydroponic system was successfully optimized, enabling large-scale experiments under sterile conditions. This system facilitates the application of chemicals in a solution and their efficient absorption by roots for molecular, biochemical, and physiological studies.

Tags

Hydroponic SystemIn VitroSterile ConditionsPlant ResponsesSmall MoleculesArabidopsis ThalianaMS MediumLaminar Flow HoodSterilizationUV LightSolid MediumLiquid Medium

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