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局部钙离子梯度对大泡囊膜重塑的响应
JoVE 杂志
化学
This content is Free Access.
JoVE 杂志 化学
Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
DOI:

08:15 min

July 16, 2018

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Chapters

  • 00:04Title
  • 00:41Preparation of Small Lipid Vesicles
  • 02:38Preparation of GUV-MLV Complexes
  • 04:12Micropipette Preparation and Microinjection
  • 06:09Formation and Translation of Membrane Tubular Protrusions (MTPs) using the Calcium Ion Source
  • 06:57Results: Formation of Membrane Tubular Protrusions in Giant Vesicles Upon Localized Application of Calcium Ion Gradients
  • 07:37Conclusion

Summary

自动翻译

我们提出了一种非接触微操作系统泡的技术, 利用局部钙离子梯度。在巨大的脂质囊的附近注射钙离子溶液, 用于重塑脂质膜, 导致膜管状突起的产生。

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