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أسلوب مبسط وفعال لعزل الخلايا الكيراتينيه البشرية الأولية من أنسجة الجلد الكبار
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发育生物学
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JoVE 杂志 发育生物学
A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
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أسلوب مبسط وفعال لعزل الخلايا الكيراتينيه البشرية الأولية من أنسجة الجلد الكبار

9,699 Views

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06:17 min

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August 25, 2018

DOI:

10.3791/57784-v

DOI:
10.3791/57784-v

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06:17 min
August 25, 2018

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3 Views
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1Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology,Shandong University, 2Suzhou Institute of Shandong University, 3Department of Urology,Qilu Hospital of Shandong University

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Primary Human KeratinocytesIsolationCultureSimplified MethodEfficientEpidermal CellsLaboratory ApplicationsClinical ApplicationsHomogenizationEnzymatic DigestionTrypsinDNase ICell StrainerCell CultureROCK InhibitorKeratinocyte MediumPassaging

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Liu, Z., Wen, J., Leng, X., Zhou, Q., Zhou, C., Zhao, H., Wu, X. A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue. J. Vis. Exp. (138), e57784, doi:10.3791/57784 (2018).

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