Journal
/
生物学
/
Diagonal Method to Measure Synergy Among Any Number of Drugs
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Diagonal Method to Measure Synergy Among Any Number of Drugs

Diagonal Method to Measure Synergy Among Any Number of Drugs

DOI:
10.3791/57713-v

•

12:08 min

•

June 21, 2018

•

, , ,
1Faculty of Engineering and Natural Sciences,Sabanci University, 2Nanotechnology Research and Application Center,Sabanci University, 3Laboratory of Systems Pharmacology,Harvard Medical School

Chapters

  • 00:04Title
  • 00:28Serial Dilution Dose-Response Experiment
  • 04:29Linear Dilution Dose-Response Experiment
  • 07:21Diagonal Drug Interaction Experiment and Interaction Scores
  • 09:51Results: Analysis of the Diagonal Drug Interaction Experiment
  • 11:11Conclusion

Summary

自动翻译

自动翻译

In this protocol, we describe how to make Loewe additivity-based drug interaction measurements for pairwise and three-way drug combinations.

Tags

Diagonal MethodSynergyMultiple DrugsSystems BiologyAntibiotic AliquotsBacterial CultureSerial DilutionsLB MediaDMSOLEVNALPNGOD600Dose-response ExperimentIC50

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

A Behavioral Assay to Measure Responsiveness of Zebrafish to Changes in Light Intensities

Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells

Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Using plusTipTracker Software to Measure Microtubule Dynamics in Xenopus laevis Growth Cones

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds

Read Article