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Elektrochemische detectie van Deuterium kinetisch-isotoopeffect op extracellulaire elektronentransport in Shewanella oneidensis heer-1
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
DOI:

09:00 min

April 16, 2018

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Chapters

  • 00:05Title
  • 01:00Formation of a Monolayer Biofilm of S. oneidensis MR-1 on an ITO Electrode
  • 04:11Replacement of the Supernatant with Fresh DM Medium with 10 mM Lactate
  • 05:24Addition of Deuterium Water to Measure the KIE on the EET Process
  • 06:26Results: Microbial Current Change Induced by D2O Addition and Confirmation of Rate-limitation by Extracellular Electron Transport Process
  • 07:36Conclusion

Summary

自动翻译

Hier presenteren we een protocol van geheel-cel elektrochemische experimenten te bestuderen van de bijdrage van proton vervoer aan het tarief van extracellulaire elektronentransport via de buitenmembraan cytochromes complex in Shewanella oneidensis heer-1.

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