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Journal / Neuroscience / Recording and Modulation of Epileptiform Activity …
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Neuroscience

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JoVE Journal Neuroscience
Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays
 

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

Article DOI: 10.3791/57548-v • 10:24 min • May 15th, 2018
May 15th, 2018 •
1, 1, 1,2
1Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 2Rehab Technologies, Istituto Italiano di Tecnologia

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We illustrate how to perform recording and electrical modulation of 4-aminopyridine-induced epileptiform activity in rodent brain slices using microelectrode arrays. A custom recording chamber maintains tissue viability throughout prolonged experimental sessions. Live electrode mapping and selection of stimulating pairs are performed by a custom graphical user interface.

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Recording Modulation Epileptiform Activity Rodent Brain Slices Microelectrode Arrays Neuromodulation Epilepsy Treatment Brain Area Stimulation Protocol Advantage Technique Rodent Brain Circuits Observation Points Inter-electrode Distance MEA (microelectrode Array) Elastomeric Sealant Recording Chamber Bubbles Tweezers Sealant Layer Moist Environment Cure At Room Temperature Overnight Recording Bench Warm Bath Holding ACSF (artificial Cerebrospinal Fluid) Pre-warming Chambers Oxygen And Carbon Dioxide Mixture Inverted Pasteur Pipette Room Temperature Holding Chamber Hippocampus-cortex Brain Slices Recovery Period
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