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Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy
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08:56 min
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May 11, 2018
DOI:
1Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Sciences,National Defense Medical Center, 2Institute of Molecular Biology,Academia Sinica, 3Institute of Biotechnology, College of Bio-Resources and Agriculture,National Taiwan University
Summary
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Here, we describe a protocol for visualizing motor neuron projection and axon arborization in transgenic Hb9::GFP mouse embryos. After immunostaining for motor neurons, we used light sheet fluorescence microscopy to image embryos for subsequent quantitative analysis. This protocol is applicable to other neuron navigation processes in the central nervous system.
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Liau, E. S., Yen, Y., Chen, J. Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy. J. Vis. Exp. (135), e57546, doi:10.3791/57546 (2018).
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